How much bacteriostatic water is typically used to reconstitute a 500 mg NAD+ vial?

Common reconstitution volumes are 1–3 mL of bacteriostatic water. At 2 mL, a 500 mg vial yields 250 mg/mL; more water lowers the concentration, which makes small amounts easier to measure on a U-100 syringe. This is a laboratory handling calculation, not medical or dosing advice.

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NAD+ Reconstitution Calculator

Pick your NAD+ vial size, enter the bacteriostatic water volume, and get the concentration and the exact draw on a U-100 insulin syringe. The coenzyme every cell runs on, in a high-mg research vial.

Vial sizes we carry:500 mg

Peptide in vial

Amount per draw

Bacteriostatic water added

Most 3 mL vials comfortably hold up to ~2.5 mL of water.

Syringe size

Draw on a U-100 syringe

0.1 units

= 0.001 mL per draw

Concentration

250 mg/mL

Draws per vial

≈ 2,000

Per draw

0.001 mL

Vial lasts

2,000 draws total

A 0.1-unit draw is hard to measure accurately. Add more water to make each draw larger.

For laboratory research calculations only, not medical, veterinary, or human-dosing advice. All Boulder Labs products are for research use only.

NAD+ concentration quick reference

Resulting concentration by vial size and bacteriostatic water volume. More water = lower concentration = more syringe units per measured amount, which is easier to read accurately on a U-100 syringe.

Vial	1 mL water	2 mL water	3 mL water
500 mg	500 mg/mL	250 mg/mL	166.67 mg/mL

NAD+ vials NAD+ research overview Reconstitution guide

For laboratory research use only. Not for human or animal consumption. This tool computes concentrations and volumes for research handling, it is not medical or dosing advice.