Reconstitution Calculator
Works in either direction. Know your water volume and want the draw? It returns the concentration and exactly how many units to pull on a U-100 syringe. Know the amount and the draw size you want? Switch to “Water to add” and it tells you how much bacteriostatic water to reconstitute with.
Most 3 mL vials comfortably hold up to ~2.5 mL of water.
= 0.05 mL per draw
For laboratory research calculations only, not medical, veterinary, or human-dosing advice. All Boulder Labs products are for research use only.
Compound-specific calculators
Each opens prefilled with that compound's vial sizes and a concentration reference table.
<iframe src="https://boulderlabs.co/embed/calculator" width="100%" height="640" style="border:1px solid #ddd;border-radius:8px" title="Peptide Reconstitution Calculator" loading="lazy"></iframe> <p style="font-size:12px"><a href="https://boulderlabs.co/calculator">Peptide reconstitution calculator</a> by Boulder Labs</p>
How to reconstitute a peptide
Reconstitution is the process of dissolving a lyophilized (freeze-dried) peptide back into liquid with bacteriostatic water. Work clean and gentle. The steps below are the standard handling routine.
Let the lyophilized peptide vial and your bacteriostatic water sit out until they reach room temperature. This reduces condensation and clumping.
Wipe the rubber stopper on the peptide vial and the bacteriostatic water vial with a fresh alcohol swab. Let them air-dry.
Use the calculator above to pick a water volume. More water = lower concentration = more units per draw (easier to measure small amounts). Draw that volume of bacteriostatic water into a syringe.
Insert the needle and let the water run slowly down the inside wall of the peptide vial. Never spray it directly onto the powder. Aggressive streams can denature the peptide.
Gently swirl or roll the vial until the powder fully dissolves into a clear solution. Shaking creates foam and can damage the peptide. If it won't dissolve, let it sit a few minutes and swirl again.
Write the date and the diluent volume on the vial so the concentration is reproducible. Store reconstituted peptide at 2–8 °C (refrigerated) and protect it from light. Use within ~2–4 weeks.
For laboratory research use only. Not for human or animal consumption. The calculator and this guide describe handling procedure, not medical or dosing advice.